Recombinant Human/Mouse Wnt-5a Protein, His Tag
产品参数
|
别名（Synonyms） |
Wingless-type MMTV Integration Site Family, Member 5a; wingless-type MMTV integration site family, member 5A; Wnt5a |
|
表达系统（Source） |
HEK293 Cells |
|
表达序列（Sequence） |
Gln38-Lys380 |
|
Accession |
P41221 |
|
分子量大小（Molecular Weight） |
The protein has a predicted MW of 40kDa. The protein migrates as 60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation |
|
纯度（Purity） |
> 90% by SDS-PAGE. |
|
内毒素（Endotoxin） |
< 1.0 EU per 1μg of the protein by the LAL method. |
|
生物活性（Biological Activity） |
Measured by its ability to inhibit Wnt-3a-induced a TCF/LEF luciferase reporter expression by 293F cells. The ED50 for this effect is 2-10µg/mL. Optimal concentrations should be determined by each laboratory for each application |
|
制剂（Formulation） |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 5% trehalose and 0.01 % Tween 80 were added as protectant before lyophilization |
|
标签（Tag） |
His tag at the C-terminal |
|
外观（Physical Appearance） |
冻干粉 |
|
复溶（Reconstitution） |
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
储存条件
-85~-65℃保存，收到货之后有效期1年。
避免反复冻融，打开小瓶前先离心。建议等分分装为一次实验使用量，分装后，-80℃冻存。
使用方法
开盖前，先离心。在含有至少0.1%人或牛血清白蛋白的无菌PBS中复溶，复溶到100μg/mL的浓度。
注意事项
1、为了您的安全和健康，请穿实验服并戴一次性手套操作。
2、本产品仅作科研用途。
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