Recombinant Human Heparin-binding EGF-like Growth Factor (Human HB-EGF)

产品参数

别名(Synonyms)

HBEGF, DT-R

Accession

Q99075

GeneID

1839

表达系统(Source)

E.coli-derived Human HB-EGF, Asp63-Leu148.

分子量大小(Molecular Weight)

Approximately 9.7 kDa.

氨基酸序列(AA Sequence)

DLQEADLDLL RVTLSSKPQA LATPNKEEHG KRKKKGKGLG KKRDPCLRKY KDFCIHGECK YVKELRAPSC ICHPGYHGER CHGLSL

标签(Tag)

None

外观(Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

纯度(Purity)

> 97% by SDS-PAGE and HPLC analyses.

生物活性(Biological Activity)

The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/mL, corresponding to a specific activity of > 1.0 × 106 IU/mg. Fully biologically active when compared to standard.

内毒素(Endotoxin)

< 1.0 EU per 1μg of the protein by the LAL method.

制剂(Formulation)

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl.

复溶(Reconstitution)

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

 

储存条件

产品用冰袋装运,可在-20℃至-80℃下保存1年。

建议第一次使用时将蛋白质分成较小的量,并避免反复冻融循环。

 

注意事项

1.避免反复冻融循环。

2.为了您的安全和健康,请穿实验服,戴一次性手套进行操作。

3.仅供研究使用!

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