Recombinant Mouse RANKL/sRANK Ligand/TNFSF11 Protein,His Tag

别名（Synonyms）

soluble Receptor Activator of NF-κB Ligand, TNFSF11, OPGL, ODF

TRANCE (TNF-related activation-induced cytokine)

Accession

O35235 :Pro143-Asp316

表达系统（Source）

Expressed in E. coli.

分子量大小（Molecular Weight）

Approximately 19.4kDa, a single non-glycosylated polypeptide chain containing 174 amino acids.

纯度（Purity）

> 96% by SDS-PAGE.

生物活性（Biological Activity）

Fully biologically active when compared to standard. Determined by its dose-dependent ability to induce reporter gene in HT-29 NF-κB Luc reporter cells, The ED50 for this effect is 0.52 ng/ml.

内毒素（Endotoxin）

＜0.01EU/μg rMusRANKL protein as determined by LAL method.

制剂（Formulation）

Lyophilized from a 0.2µm filtered concentrated solution in 20mMPB, 300mM NaCl, pH7.4.

复溶（Reconstitution）

Before use this product, please read the direction below carefully.

1.This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.

2.Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml.

Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.