Recombinant Canine Stem Cell Factor (Canine SCF)

别名（Synonyms）

Clo Protein, Con Protein, Gb Protein, Kitlg Protein, Mgf Protein, SCF Protein, SF Protein, Sl Protein, SLF Protein

Accession

Q06220

GeneID

403507

表达系统（Source）

E.coli-derived canine Stem Cell Factor, Lys26-Ala190

分子量大小（Molecular Weight）

Approximately 18.4 kDa.

氨基酸序列(AA Sequence)

KGICGKRVTD DVKDVTKLVA NLPKDYKIAL KYVPGMDVLP SHCWISVMVE QLSVSLTDLL DKFSNISEGL SNYSIIDKLV KIVDDLVECT EGYSFENVKK APKSPELRLF TPEEFFRIFN RSIDAFKDLE TVASKSSECV VSSTLSPDKD SRVSVTKPFM LPPVA

标签（Tag）

None

外观（Physical Appearance）

Sterile Filtered White lyophilized (freeze-dried) powder.

纯度（Purity）

> 96 % by SDS-PAGE and HPLC analyses.

生物活性（Biological Activity）

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using human TF-1 cells is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 × 10⁵ IU/mg.

内毒素（Endotoxin）

< 1.0 EU per 1μg of the protein by the LAL method.

制剂（Formulation）

Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4.

复溶（Reconstitution）

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.