Recombinant Mouse NGF Protein
产品参数
|
别名（Synonyms） |
beta Nerve Growth Factor; Ngfb |
| Uniprot No. | NP_001106168.1 |
|
表达系统（Source） |
Recombinant Mouse NGF Protein is expressed from CHO Stable Cells Cells with an initial Met at the C-terminus. It contains Ser 122-Gly 241. |
|
分子量大小（Molecular Weight） |
The recombinant mouse NGF has a calculated molecular mass of 13.5 kDa as estimated in SDS-PAGE under reducing conditions. |
|
纯度（Purity） |
> 95% as determined by SDS-PAGE. |
|
生物活性（Biological Activity） |
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 1-6 ng/mL. |
|
内毒素（Endotoxin） |
<1 EU per 1μg of the protein by the LAL method. |
|
制剂（Formulation） |
Lyophilized from sterile 150mM NaCl, 50mM NaAc, pH 5.5.Normally 5% - 8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
|
复溶（Reconstitution） |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. |
储存条件
产品用冰袋装运，可在-20℃至-80℃下保存1年。
建议第一次使用时将蛋白质分成较小的量，并避免反复冻融循环。
注意事项
1.避免反复冻融循环。
2.为了您的安全和健康，请穿实验服，戴一次性手套进行操作。
3.仅供研究使用！
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