Recombinant Human Neurotrophin 3(Human NT-3)
产品参数
|
别名（Synonyms） |
HDNF ; NGF-2 ; NGF2 ; NT-3; NT3 |
|
Accession |
P20783-1 |
|
表达系统（Source） |
E. coli-derived human NT-3 protein, Tyr139-Thr257 |
|
分子量大小（Molecular Weight） |
Approximately 13.8 kDa. |
|
氨基酸序列(AA Sequence) |
YA EHKSHRGEYS VCDSESLWVT DKSSAIDIRG HQVTVLGEIK TGNSPVKQYF YETRCKEARP VKNGCRGIDD KHWNSQCKTS QTYVRALTSE NNKLVGWRWI RIDTSCVCAL SRKIGRT |
|
标签（Tag） |
None |
|
外观（Physical Appearance） |
Sterile Filtered White lyophilized (freeze-dried) powder. |
|
纯度（Purity） |
> 90% by SDS-PAGE. |
|
生物活性（Biological Activity） |
Measured by its binding ability in a functional ELISA. Immobilized human NT3 at 2 μg/mL (100 μL/well) can bind human TrkB-Fch. The EC50 of human TrkB-Fch is 120-350 ng/mL. |
|
内毒素（Endotoxin） |
< 1.0 EU per 1μg of the protein by the LAL method. |
|
制剂（Formulation） |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 5.2. |
|
复溶（Reconstitution） |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
储存条件
产品用冰袋装运，可在-20℃至-80℃下保存1年。
建议第一次使用时将蛋白质分成较小的量，并避免反复冻融循环。
注意事项
1.避免反复冻融循环。
2.为了您的安全和健康，请穿实验服，戴一次性手套进行操作。
3.仅供研究使用！
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