产品参数
别名(Synonyms) |
Neurotrophin-5, NT-5; Neurotrophin-4 ;GLC10 |
Accession |
P34130 |
GeneID |
4909 |
表达系统(Source) |
E.coli-derived Human NT-4, G81-A210, with an N-terminal Met. |
分子量大小(Molecular Weight) |
Approximately 28.1 kDa. |
氨基酸序列(AA Sequence) |
MGVSETAPAS RRGELAVCDA VSGWVTDRRT AVDLRGREVE VLGEVPAAGG SPLRQYFFET RCKADNAEEG GPGAGGGGCR GVDRRHWVSE CKAKQSYVRA LTADAQGRVG WRWIRIDTAC VCTLLSRTGR A |
标签(Tag) |
None |
外观(Physical Appearance) |
Sterile Filtered White lyophilized (freeze-dried) powder. |
纯度(Purity) |
> 97 % by SDS-PAGE and HPLC analyses. |
生物活性(Biological Activity) |
The ED50 as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures is less than 50 ng/mL, corresponding to a specific activity of > 2.0 × 104 IU/mg. Fully biologically active when compared to standard. |
内毒素(Endotoxin) |
< 1.0 EU per 1μg of the protein by the LAL method. |
制剂(Formulation) |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 5.5. |
复溶(Reconstitution) |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
储存条件
产品用冰袋装运,可在-20℃下保存1年。 复溶后在无菌条件下2至8°C保存1个月。 复溶后在-20°C无菌条件下保存3个月。 建议第一次使用时将蛋白质分成较小的量,并避免反复冻融循环。
注意事项
1.避免反复冻融循环。
2.为了您的安全和健康,请穿实验服,戴一次性手套进行操作。
3.仅供研究使用!
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