Recombinant Mouse VEGF 164 Protein

产品参数

别名(Synonyms)

Vascular Endothelial Growth Factor Isoform 164

Accession

Q00731

GeneID

Mm.282184.

表达系统(Source)

Yeast-derived mouse VEGF164 protein, Ala27-Arg190 (N115K), with Met at N-terminus.

分子量大小(Molecular Weight)

Approximately 40 kDa in SDS-PAGE under non-reducing conditions.

氨基酸序列(AA Sequence)

MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR

标签(Tag)

None

外观(Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

纯度(Purity)

> 97% by SDS-PAGE and HPLC analyses.

生物活性(Biological Activity)

The ED50 as determined by a cell proliferation assay using human umbilical veinendothelial cells(HUVEC) is between 1.0-5.0 ng/mL. Fully biologically active when compared to standard.

内毒素(Endotoxin)

< 0.01 EU per 1μg of the protein by the LAL method.

制剂(Formulation)

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

复溶(Reconstitution)

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20. Further dilutions should be made in appropriate buffered solutions.

 

储存条件

产品用冰袋装运,可在-20 ℃下保存1年。 复溶后在无菌条件下2至8°C保存1个月。 复溶后在-20°C无菌条件下保存3个月。 建议第一次使用时将蛋白质分成较小的量,并避免反复冻融循环。

 

注意事项

1.避免反复冻融循环。

2.为了您的安全和健康,请穿实验服,戴一次性手套进行操作。

3.仅供研究使用!

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