Recombinant Human PDGF-CC Protein,His Tag

产品参数

别名(Synonyms)

PDGFCC; PDGF-CC

表达系统(Source)

E.coli

分子量大小(Molecular Weight)

Approximately 27 kDa, a disulfide-linked homodimer of two 117 amino acid, C-terminal polyhistidine tagged proteins.

氨基酸序列(AA Sequence)

Accession :NP_057289 Val235-Gly345,  with an N-terminal Met and 6-His tag;

纯度(Purity)

> 97% by SDS-PAGE.

生物活性(Biological Activity)

Measured in a cell proliferation assay using NR6R3T3 mouse fibroblast cells. The ED50 for this effect is 70-350 ng/mL.

内毒素(Endotoxin)

<0.1EU per 1μg of the protein by the LAL method.

制剂(Formulation)

Lyophilized from 0.2 µm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.

复溶(Reconstitution)

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4 mM HCl to a concentration of 0.1 mg/mL. Further dilutions should be made in appropriately buffered solutions.

 

储存条件

该产品应在-25~-15℃下储存一年,从收到之日起算。 复溶后在无菌条件下2~8°C保存1个月。 复溶后在-25~-15℃无菌条件下保存3个月。 建议第一次使用时将蛋白质分成较小的量,并避免反复冻融循环。

 

注意事项

1.避免反复冻融循环。

2.为了您的安全和健康,请穿实验服,戴一次性手套进行操作。

3.仅供研究使用!

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