产品参数
别名(Synonyms) |
Insulin-like Growth Factor-II, Somatamedin A |
Accession |
E3UN46 |
GeneID |
3481 |
表达系统(Source) |
E.coli-derived Human IGF-2 protein,Ala25-Glu91. |
分子量大小(Molecular Weight) |
Approximately 7.5 kDa. |
氨基酸序列(AA Sequence) |
AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE |
标签(Tag) |
None |
外观(Physical Appearance) |
Sterile Filtered White lyophilized (freeze-dried) powder. |
纯度(Purity) |
> 98% by SDS-PAGE and HPLC analyses. |
生物活性(Biological Activity) |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/mL, corresponding to a specific activity of > 5.0 × 105 IU/mg. |
内毒素(Endotoxin) |
< 1.0 EU per 1μg of the protein by the LAL method. |
制剂(Formulation) |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 150 mM NaCl, with 0.02 % Tween-20. |
复溶(Reconstitution) |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
储存条件
产品用冰袋装运,可在-20℃至-80℃下保存1年。
建议第一次使用时将蛋白质分成较小的量,并避免反复冻融循环。
注意事项
1.避免反复冻融循环。
2.为了您的安全和健康,请穿实验服,戴一次性手套进行操作。
3.仅供研究使用!
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