产品参数
别名(Synonyms) |
LR3 IGF-I, IBP1, IGF1, IGF-1, IGF1A, IGFI, IGF-I, IGF-IA, IGF-IB, MGF |
Accession |
P05019 |
GeneID |
3479 |
表达系统(Source) |
E.coli-derived human LR3 IGF-1 protein, Gly49-Ala118. |
分子量大小(Molecular Weight) |
Approximately 9.1 kDa. |
氨基酸序列(AA Sequence) |
MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA KSA |
标签(Tag) |
None |
外观(Physical Appearance) |
Sterile Filtered White lyophilized (freeze-dried) powder. |
纯度(Purity) |
>96% by SDS-PAGE analyses. |
生物活性(Biological Activity) |
1.Fully biologically active when compared to standard. Measured in a serum-free cell proliferation assay using human MCF-7 cells. The ED50 for this effect is 0.3-1.5 ng/mL, corresponding to a specific activity of > 6.7 × 105 U/mg. 2.The ED50 as determined by the stimulation of protein synthesis using rat L6 myoblasts is less than 10 ng/mL, corresponding to a specific activity of > 1.0 × 105 U/mg. Fully biologically active when compared to standard. |
内毒素(Endotoxin) |
< 0.01 EU per 1μg of the protein by the LAL method. |
制剂(Formulation) |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.2. |
复溶(Reconstitution) |
Before use this product, please read the direction below carefully. 1.This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. 2.Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
储存条件
该产品应在-25~-15℃下储存一年,从收到之日起算。 复溶后在无菌条件下2~8°C保存1个月。 复溶后在-25~-15℃无菌条件下保存3个月。 建议第一次使用时将蛋白质分成较小的量,并避免反复冻融循环。
注意事项
1.避免反复冻融循环。
2.为了您的安全和健康,请穿实验服,戴一次性手套进行操作。
3.仅供研究使用!
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