产品参数
别名(Synonyms) |
soluble TRAIL Receptor-2, DR5, TNFRSF10B, KILER, TRICK2A, TRICKB |
Accession |
O14763 |
GeneID |
8795 |
表达系统(Source) |
E.coli-derived Human soluble TRAIL Receptor-2 protein,Glu52-Ser183. |
分子量大小(Molecular Weight) |
Approximately 14.8 kDa |
氨基酸序列(AA Sequence) |
ESALITQQDL APQQRAAPQQ KRSSPSEGLC PPGHHISEDG RDCISCKYGQ DYSTHWNDLL FCLRCTRCDS GEVELSPCTT TRNTVCQCEE GTFREEDSPE MCRKCRTGCP RGMVKVGDCT PWSDIECVHK ES |
标签(Tag) |
None |
外观(Physical Appearance) |
Sterile Filtered White lyophilized (freeze-dried) powder. |
纯度(Purity) |
> 97 % by SDS-PAGE and HPLC analyses. |
生物活性(Biological Activity) |
Fully biologically active when compared to standard. rHusTRAIL-R2 reduced the production of LPS-induced TNF by its ability to neutralize endogenous TRAIL in fresh human PBMC. In this assay, endogenous TRAIL is induced during a 24 hour exposure to LPS (10 ng/mL) but in the presence of rHusTRAIL-R2, TRAIL-induced TNF is suppressed. |
内毒素(Endotoxin) |
< 1.0 EU per 1μg of the protein by the LAL method. |
制剂(Formulation) |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
复溶(Reconstitution) |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
储存条件
产品用冰袋装运,可在-20℃下保存1年。 复溶后在无菌条件下2至8°C保存1个月。 复溶后在-20°C无菌条件下保存3个月。 建议第一次使用时将蛋白质分成较小的量,并避免反复冻融循环。
注意事项
1.避免反复冻融循环。
2.为了您的安全和健康,请穿实验服,戴一次性手套进行操作。
3.仅供研究使用!
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