Recombinant Human TNF-related Weak Inducer of Apoptosis Receptor/TNFRSF12A (Human TWEAK Re

产品参数

别名(Synonyms)

TNFRSF12A, FGF-inducible 14, CD266

Accession

Q9NP84

GeneID

51330

表达系统(Source)

E.coli-derived Human TWEAK Receptor/TNFRSF12A protein,Glu28-Pro80.

分子量大小(Molecular Weight)

Approximately 5.6 kDa

氨基酸序列(AA Sequence)

EQAPGTAPCS RGSSWSADLD KCMDCASCRA RPHSDFCLGC AAAPPAPFRL LWP

标签(Tag)

None

外观(Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

纯度(Purity)

> 95% by SDS-PAGE and HPLC analyses.

生物活性(Biological Activity)

The ED50 as determined by inhibiting TWEAK-dependent proliferation of human umbilical vein endothelial cells (HUVEC) is less than 30 ng/ml, corresponding to a specific activity of > 3.3 × 104 IU/mg, in the presence of 15 ng/mL of rHuTWEAK. Fully biologically active when compared to standard.

内毒素(Endotoxin)

< 1.0 EU per 1μg of the protein by the LAL method.

制剂(Formulation)

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

复溶(Reconstitution)

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

 

储存条件

产品用冰袋装运,可在-20℃至-80℃下保存1年。

建议第一次使用时将蛋白质分成较小的量,并避免反复冻融循环。

 

注意事项

1.避免反复冻融循环。

2.为了您的安全和健康,请穿实验服,戴一次性手套进行操作。

3.仅供研究使用!

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