Recombinant Human/Murine/Rat Activin A Protein

产品参数

别名(Synonyms)

Activin A, activin AB alpha polypeptide, inhibin beta A chain
Uniprot No. P08476

表达系统(Source)

Human/Murine/Rat Activin A Protein is expressed from CHO cell.It contains Gly311 - Ser426.

分子量大小(Molecular Weight)

Apparent molecular mass of 24 kDa in SDS-PAGE under non-reducing conditions, 14 kDa under reducing conditions, a disulfide-linked homodimer of two 116 amino acid glycosylated polypeptide chains.

纯度(Purity)

> 95% as determined by SDS-PAGE.

生物活性(Biological Activity)

Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. The ED50 for this effect is 0.2-1.2 ng/mL.The specific activity of Activin A is approximately 1 IU/μg.

内毒素(Endotoxin)

<0.01 EU per 1μg of the protein by the LAL method.

制剂(Formulation)

Lyophilized from 0.2 μm filtered concentrated solution in 30 % acetonitrile and 0.1 % TFA.

复溶(Reconstitution)

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile 4 mM HCl to a concentration of 0.1-0.5 mg/ml. Further dilutions should be made in appropriately buffered solutions.

 

储存条件

该产品应在-25~-15℃下储存一年,从收到之日起算。

复溶后在无菌条件下2~8°C保存1个月。

复溶后在-25~-15℃无菌条件下保存3个月。

建议第一次使用时将蛋白质分成较小的量,并避免反复冻融循环。

 

注意事项

1.为了您的安全和健康,请穿实验服,戴一次性手套进行操作。

2.仅供研究使用!

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