产品参数
别名(Synonyms) |
Activin A, activin AB alpha polypeptide, inhibin beta A chain |
Uniprot No. | P08476 |
表达系统(Source) |
Human/Murine/Rat Activin A Protein is expressed from CHO cell.It contains Gly311 - Ser426. |
分子量大小(Molecular Weight) |
Apparent molecular mass of 24 kDa in SDS-PAGE under non-reducing conditions, 14 kDa under reducing conditions, a disulfide-linked homodimer of two 116 amino acid glycosylated polypeptide chains. |
纯度(Purity) |
> 95% as determined by SDS-PAGE. |
生物活性(Biological Activity) |
Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. The ED50 for this effect is 0.2-1.2 ng/mL.The specific activity of Activin A is approximately 1 IU/μg. |
内毒素(Endotoxin) |
<0.01 EU per 1μg of the protein by the LAL method. |
制剂(Formulation) |
Lyophilized from 0.2 μm filtered concentrated solution in 30 % acetonitrile and 0.1 % TFA. |
复溶(Reconstitution) |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile 4 mM HCl to a concentration of 0.1-0.5 mg/ml. Further dilutions should be made in appropriately buffered solutions. |
储存条件
该产品应在-25~-15℃下储存一年,从收到之日起算。
复溶后在无菌条件下2~8°C保存1个月。
复溶后在-25~-15℃无菌条件下保存3个月。
建议第一次使用时将蛋白质分成较小的量,并避免反复冻融循环。
注意事项
1.为了您的安全和健康,请穿实验服,戴一次性手套进行操作。
2.仅供研究使用!
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