Recombinant Mouse M-CSF Protein
产品描述
Monocyte colony-stimulating factor (M-CSF, CSF-1) is a macrophage lineage-specific growth factor. Full length human M-CSF transcripts encode a 522 amino acid (aa) type I transmembrane (TM) protein with a 464 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. The first 223 aa of mature human M-CSF shares 88%, 86%, 81% and 74% aa identity with corresponding regions of dog, cow, mouse and rat M CSF, respectively. M-CSF Induces vascular endothelial growth factor production and angiogenic activity from human monocytes. Whatmore, M-CSF and GM-CSF are mediators involved in regulating the numbers and function of macrophage lineage populations and have been shown to contribute to macrophage heterogeneity.
产品参数
|
别名（Synonyms） |
CSF-1, MGI-IM, MCSF, M-CSF, MGC31930 |
|
Accession |
Q3U4F9 |
|
UniGene |
Mm.795. |
|
表达系统（Source） |
E.coli-derived Mouse M-CSF protein, Lys33-Glu262. |
|
分子量大小（Molecular Weight） |
Approximately 52.0 kDa. |
|
氨基酸序列(AA Sequence) |
KEVSEHCSHM IGNGHLKVLQ QLIDSQMETS CQIAFEFVDQ EQLDDPVCYL KKAFFLVQDI IDETMRFKDN TPNANATERL QELSNNLNSC FTKDYEEQNK ACVRTFHETP LQLLEKIKNF FNETKNLLEK DWNIFTKNCN NSFAKCSSRD VVTKPDCNCL YPKATPSSDP ASASPHQPPA PSMAPLAGLA WDDSQRTEGS SLLPSELPLR IEDPGSAKQR PPRSTCQTLE |
|
标签（Tag） |
None |
|
外观（Physical Appearance） |
Sterile Filtered White lyophilized (freeze-dried) powder. |
|
纯度（Purity） |
> 95% by SDS-PAGE and HPLC analyses. |
|
生物活性（Biological Activity） |
The ED50 as determined by a cell proliferation assay using murine M-NFS-60 cells is less than 2 ng/mL, corresponding to a specific activity of > 5.0 × 105 IU/mg. Fully biologically active when compared to standard. |
|
内毒素（Endotoxin） |
< 1.0 EU per 1μg of the protein by the LAL method. |
|
制剂（Formulation） |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris, 500 mM NaCl, pH 7.4. |
|
复溶（Reconstitution） |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
储存条件
产品用冰袋装运，可在-20 ℃下保存1年。
复溶后在无菌条件下2至8°C保存1个月。
复溶后在-20°C无菌条件下保存3个月。
建议第一次使用时将蛋白质分成较小的量，并避免反复冻融循环。
注意事项
1.避免反复冻融循环。
2.为了您的安全和健康，请穿实验服，戴一次性手套进行操作。
3.仅供研究使用！
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