Porcine IL-1RA
产品描述
IL-1ra was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The naturally-occurring, fully glycosylated form has an apparent molecular weight of about 25,000 Daltons. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha. It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptors (I and II) on human cells, but reportedly will not block binding to the type II receptor on murine pre-B cell lines. The recombinant, non-glycosylated form of IL-ra blocks binding of IL-1 to its receptor equally as well as the naturally-occurring, glycosylated form. The IL-1ra has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Human, mouse and rat IL-1ra all share approximately 80% homology with porcine IL-1ra.
产品参数
|
别名（Synonyms） |
DIRA; ICIL-1ra; IL1F3 |
|
Accession |
Q29056 |
|
GeneID |
397499 |
|
表达系统（Source） |
E.coli-derived Porcine IL-1RA, His26-Gln177. |
|
分子量大小（Molecular Weight） |
E.coli-derived Mouse IL-36RA, Val3-Asp156. |
|
氨基酸序列(AA Sequence) |
HPLGKRPCRM QAFRIWDVNQ KTFYLRNNQL VAGYLQGPNT KLEEKIDVVP VEPHFVFLGI HGGKLCLSCV KSGDEMKLQL DAVNITDLRK NSEQDKRFTF IRSDSGPTTS FESAACPGWF LCTALEADQP VGLTNTPKAA VKVTKFYFQQ DQ |
|
标签（Tag） |
None |
|
外观（Physical Appearance） |
Sterile Filtered White lyophilized (freeze-dried) powder. |
|
纯度（Purity） |
> 97% by SDS-PAGE and HPLC analyses. |
|
生物活性（Biological Activity） |
The ED50 as determined by inhibiting IL-1α-dependent proliferation of murine D10S cells is less than 50 ng/mL, corresponding to a specific activity of > 2.0 × 104 IU/mg in the presence of 75 pg/mL rPoIL-1α. Fully biologically active when compared to standard. |
|
内毒素（Endotoxin） |
< 0.1 EU per 1μg of the protein by the LAL method. |
|
制剂（Formulation） |
Lyophilized from a 0.2 µm filtered concentrated solution in 1 × PBS, pH 7.4, 1 mM DTT. |
|
复溶（Reconstitution） |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
储存条件
产品用冰袋装运，可在-20℃至-80℃下保存1年。
建议第一次使用时将蛋白质分成较小的量，并避免反复冻融循环。
注意事项
1.避免反复冻融循环。
2.为了您的安全和健康，请穿实验服，戴一次性手套进行操作。
3.仅供研究使用！
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