Rat G-CSF
产品描述
Granulocyte colony stimulating factor (G-CSF) is a pleiotropic cytokine. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Human G-CSF is 73% identical at the amino acid level to mouse G-CSF and the two proteins show species cross-reactivity. G-CSF receptor was expressed on cardiomyocytes and G-CSF activated the Jak/Stat pathway in cardiomyocytes. The G-CSF treatment did not affect initial infarct size at 3 d but improved cardiac function as early as 1 week after myocardial infarction. And the G-CSF induces stem cell mobilization by decreasing bone marrow SDF-1 and up-regulating CXCR4.
产品参数
|
别名（Synonyms） |
G-CSF, CSF-3, MGI-1G, GM-CSF beta, MGC45931, Pluripoietin |
|
Accession |
P97712 |
|
GeneID |
25610 |
|
表达系统（Source） |
E.coli-derived rat G-CSF protein, Ile22-Ile214. |
|
分子量大小（Molecular Weight） |
Approximately 21.5 kDa. |
|
氨基酸序列(AA Sequence) |
KKIPLLTVSS LPPSLPLPRS FLLKSLEQVR KIQARNTELL EQLCATYKLC HPEELVLFGH SLGIPKASLS SCSSQALQQT KCLSQLHSGL FLYQGLLQAL AGISSELAPT LDMLHLDVDN FATTIWQQME SLGVAPTVQP TQSTMPIFTS AFQRRAGGVL VTSYLQSFLE TAHHALHHLP RPAQKHFPES LFISI |
|
标签（Tag） |
None |
|
外观（Physical Appearance） |
Sterile Filtered White lyophilized (freeze-dried) powder. |
|
纯度（Purity） |
> 97% by SDS-PAGE and HPLC analyses. |
|
生物活性（Biological Activity） |
The ED50 determined by a cell proliferation assay using murine NFS-60 cells is less than 0.05 ng/mL, corresponding to a specific activity of > 2.0 × 107 IU/mg. Fully biologically active when compared to standard. |
|
内毒素（Endotoxin） |
< 0.1 EU per 1μg of the protein by the LAL method. |
|
制剂（Formulation） |
Lyophilized from a 0.2 μm filtered concentrated solution in 5 mM Sodium Citrate, pH 4.0. |
|
复溶（Reconstitution） |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
储存条件
产品用冰袋装运，可在-20℃至-80℃下保存1年。
建议第一次使用时将蛋白质分成较小的量，并避免反复冻融循环。
注意事项
1.避免反复冻融循环。
2.为了您的安全和健康，请穿实验服，戴一次性手套进行操作。
3.仅供研究使用！
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