Recombinant Rat Interleukin-10 (Rat IL-10)
产品描述
Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of α-helical cytokines that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155. IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer. Mature human IL10 shares 72%86% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat IL10. IL-10, a cytokine produced by CD4+ T lymphocytes belonging to the Th-2 subset, has previously been shown to inhibit the synthesis of IFN-gamma by both T cells and NK cells. The anti-inflammatory actions of IL-10 may be therapeutically useful either directly or through modulation of HO-1 activity.
产品参数
|
别名（Synonyms） |
Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, IL10A, TGIF, CSIF |
|
Accession |
P29456 |
|
GeneID |
25325 |
|
表达系统（Source） |
E.coli-derived rat IL-10 protein, Ser19-Asn178. |
|
分子量大小（Molecular Weight） |
Approximately 18.6 kDa. |
|
氨基酸序列(AA Sequence) |
SKGHSIRGDN NCTHFPVSQT HMLRELRAAF SQVKTFFQKK DQLDNILLTD SLLQDFKGYL GCQALSEMIK FYLVEVMPQA ENHGPEIKEH LNSLGEKLKT LWIQLRRCHR FLPCENKSKA VEQVKNDFNK LQDKGVYKAM NEFDIFINCI EAYVTLKMKN |
|
标签（Tag） |
None |
|
外观（Physical Appearance） |
Sterile Filtered White lyophilized (freeze-dried) powder. |
|
纯度（Purity） |
> 97% by SDS-PAGE and HPLC analyses. |
|
生物活性（Biological Activity） |
The ED50 as determined by a cell proliferation assay using murine MC/9-2 cells is less than 1.0 ng/mL, corresponding to a specific activity of > 1.0 × 106 IU/mg. Fully biologically active when compared to standard. |
|
内毒素（Endotoxin） |
< 0.1 EU per 1μg of the protein by the LAL method. |
|
制剂（Formulation） |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 100 mM NaCl. |
|
复溶（Reconstitution） |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10 mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
储存条件
产品用冰袋装运，可在-20℃至-80℃下保存1年。
建议第一次使用时将蛋白质分成较小的量，并避免反复冻融循环。
注意事项
1.避免反复冻融循环。
2.为了您的安全和健康，请穿实验服，戴一次性手套进行操作。
3.仅供研究使用！
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