Recombinant Rat IL-4 Protein
产品描述
Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a pleiotropic cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells and produced by by mast cells, activated T cells and bone marrow stromal cells. Mature human IL-4 shares 55%, 39% and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively.It is a potent cytokine produced by T cells and to a lesser extent by tumor-associated natural killer cells, basophils, and mast cells. IL-4 is a differentiation factor for transforming growth factor-beta secreting Th3. And IL-4 activates two distinct signalling pathways through tyrosine phosphorylation of Stat6, a signal transducer and activator of transcription, and of a 170K protein called 4PS.
产品参数
|
别名（Synonyms） |
Interleukin-4, IL-4, BSF-1, BCDF, IL4E12, Pitrakinra |
|
Accession |
P20096 |
|
GeneID |
287287 |
|
表达系统（Source） |
E.coli-derived rat IL-4 protein, His23-Ser147. |
|
分子量大小（Molecular Weight） |
Approximately 14.0 kDa. |
|
氨基酸序列(AA Sequence) |
HGCNDSPLRE IINTLNQVTE KGTPCTEMFV PDVLTATRNT TENELICRAS RVLRKFYFPR DVPPCLKNKS GVLGELRKLC RGVSGLNSLR SCTVNESTLT TLKDFLESLK SILRGKYLQS CTSMS |
|
标签（Tag） |
None |
|
外观（Physical Appearance） |
Sterile Filtered White lyophilized (freeze-dried) powder. |
|
纯度（Purity） |
> 95% by SDS-PAGE and HPLC analyses. |
|
生物活性（Biological Activity） |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat splenocytes is less than 1.0 ng/mL, corresponding to a specific activity of > 1.0 × 106 IU/mg. |
|
内毒素（Endotoxin） |
< 1.0 EU per 1μg of the protein by the LAL method. |
|
制剂（Formulation） |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, 5% trehalose. |
|
复溶（Reconstitution） |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
储存条件
产品用冰袋装运，可在-20 ℃下保存1年。
复溶后在无菌条件下2至8°C保存1个月。
复溶后在-20°C无菌条件下保存3个月。
建议第一次使用时将蛋白质分成较小的量，并避免反复冻融循环。
注意事项
1.避免反复冻融循环。
2.为了您的安全和健康，请穿实验服，戴一次性手套进行操作。
3.仅供研究使用！
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