Recombinant Human Dickkopf-related protein-1,His (Human DKK-1,His )
产品参数
|
别名（Synonyms） |
Dickkopf-1;DKK-1;SK Protein; DKK1; dkk1; dkk-1 |
|
表达系统（Source） |
Human DKK1 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Thr32-His266.[Accession | O94907] |
|
分子量大小（Molecular Weight） |
The protein has a predicted MW of 26.87 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Tris-Bis PAGE result. |
|
外观（Physical Appearance） |
White freeze-dried powder |
|
纯度（Purity） |
> 95% as determined by Tris-Bis PAGE and HPLC |
|
生物活性（Biological Activity） |
ELISA Data: Immobilized Human DKK1, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-DKK1 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA. |
|
内毒素（Endotoxin） |
< 1.0 EU/μg by the LAL method. |
|
制剂（Formulation） |
Lyophilized from 0.22μm filtered solution in 20mM PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
复溶（Reconstitution） |
Centrifuge before opening the lid. Re-dissolving with deionized water until the concentration is not lower than 0.1 mg/mL. |
储存条件
-25~-15℃保存，收到货之后有效期1年。
复溶后， 2 ~8 °C保存，7天有效期。复溶后， -85~-65℃保存，3个月有效期。
建议首次使用时将蛋白等分，避免重复冻融循环。
注意事项
1.避免反复冻融循环。
2.为了您的安全和健康，请穿实验服，戴一次性手套进行操作。
3.仅供研究使用！
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